Efficacy of the combination of milbemycin oxime and afoxolaner for the control of myiasis by Musca domestica in desert tortoises (Gopherus berlandieri).

The efficacy of the combination of milbemycin oxime and afoxolaner was evaluated in desert tortoises infested with fly larvae. Oral administration of the combination of milbemycin oxime and afoxolaner eliminated the infestation without generating any evident adverse effects on the tortoises.

L'efficacité de la combinaison de milbémycine oxime et d'afoxolaner a été évaluée chez des tortues du désert infestées par des larves de mouches. L'administration orale de la combinaison de milbémycine oxime et d'afoxolaner traite l'infestation sans générer d'effets indésirables évidents sur les tortues.

A eficácia da associação de milbemicina oxima e afoxolaner foi avaliada em tartarugas do deserto infestadas por larvas de moscas. A administração oral da combinação de afoxolaner e milbemicina oxima eliminou a infestação sem gerar nenhum efeito adverso evidente às tartarugas.

Se evaluó la eficacia de la combinación de milbemicina oxima y afoxolaner en tortugas del desierto infestadas con larvas de mosca. La administración oral de la combinación de milbemicina oxima y afoxolaner eliminó la infestación sin generar efectos adversos evidentes en las tortugas.

Effectiveness of lotilaner against ticks of the genus Amblyomma spp. in three naturally infested cane toads (Rhinella horribilis).

The efficacy of lotilaner was evaluated in three tick-infested cane toads. A single oral administration of lotilaner eliminated all ticks from all three toads from Day (D)1. Environmental samples collected from toad enclosures were negative for ticks until D30.

L'efficacité du lotilaner a été évaluée chez trois crapauds buffle infestés de tiques. Une seule administration orale de lotilaner a éliminé toutes les tiques des trois crapauds à partir du jour (J)1. Les échantillons environnementaux prélevés dans les enclos des crapauds étaient négatifs pour les tiques jusqu'à J30.

Se evaluó la eficacia de lotilaner en tres sapos de caña infestados por garrapatas. Una sola administración oral de lotilaner eliminó todas las garrapatas de los tres sapos desde el día (D) 1. Las muestras ambientales recolectadas de los recintos de sapos fueron negativas para garrapatas hasta el D30.

A eficácia do lotialaner foi avaliada em três sapos-boi infestados por carrapatos. Uma única administração de lotialaner eliminou todos os carrapatos de todos os sapos no Dia (D) 1. Amostras ambientais coletadas dos terrários dos sapos foram todas negativas para carrapatos até o D30.

Dermatophytosis caused by Trichophyton benhamiae in a dog.

Trichophyton benhamiae was diagnosed in a 9-year-old female dog by histopathological evaluation, fungal culture and confirmation by sequencing of the internal transcribed spacer region of ribosomal DNA. Successful therapy was achieved with itraconazole, bathing with miconazole and chlorhexidine shampoo, and topical application of sodium hypochlorite as a rinse.

Trichophyton benhamiae a été diagnostiqué chez une chienne de 9 ans par examen histopathologique, culture fongique et confirmation par séquençage de la région ITS (internal trasbcriber spacer) de l'ADN ribosomique. Une guérison thérapeutique a été obtenue par de l'itraconazole, des shampooings de miconazole et chlorhexidine et application topique d'hypochlorite de sodium en rinçage.

Trichophyton benhamiae foi diagnosticado em uma cadela de 9 anos através de avaliação histopatológica, cultura de fungos e confirmação por sequenciamento da região espaçadora transcrita interna do DNA ribossomal. Sucesso terapêutico foi obtido com o uso de itraconazol, banho com shampoo à base de miconazol e clorexidina e aplicação tópica de hipoclorito de sódio.

Se diagnosticó infección por Trichophyton benhamiae en una perra de 9 años mediante evaluación histopatológica, cultivo de hongos y confirmación mediante secuenciación de la región espaciadora transcrita interna del DNA ribosómico. Se logró un tratamiento exitoso con itraconazol, baños con champú de miconazol y clorhexidina y aplicación tópica de hipoclorito de sodio como enjuague.

Successful treatment of Ophionyssus natricis with afoxolaner in two Burmese pythons (Python molurus bivittatus).

The efficacy of afoxolaner was evaluated in two captive Burmese python snakes, which were naturally infested with Ophionyssus natricis mites. The administration of a single oral dose of afoxolaner eliminated live O. natricis mites from both snakes by Day 3. Environmental samples collected from the snakes' terrariums were negative for dead mites by Day 30.

L'efficacité de l'afoxolaner a été évaluée chez deux serpents python birmans captifs, naturellement infestés par des acariens Ophionyssus natricis. L'administration d'une dose orale unique d'afoxolaner a éliminé les acariens O. natricis vivants des deux serpents à jour 3. Les échantillons environnementaux prélevés dans les terrariums des serpents étaient négatifs pour les acariens morts à jour 30.

Se evaluó la eficacia de afoxolaner en dos serpientes pitón birmanas cautivas, que estaban naturalmente infestadas con ácaros Ophionyssus natricis. La administración de una sola dosis oral de afoxolaner eliminó los ácaros O. natricis vivos de ambas serpientes en el día 3. Las muestras ambientales recolectadas de los terrarios de las serpientes dieron negativo para ácaros muertos en el día 30.

A eficácia do afoxolaner foi avaliada em duas cobras python birmanesas de cativeiro, que foram naturalmente infestadas com ácaros Ophionyssus natricis. A administração de uma única dose oral de afoxolaner eliminou ácaros O. natricis vivos de ambas as cobras no dia 3. As amostras ambientais coletadas dos terrários das cobras foram negativas para ácaros mortos no dia 30.

Use of afoxolaner for the treatment of lice (Goniodes pavonis) in different genera (Chrysolophus spp, Lophura spp, Phasianus spp, and Syrmaticus spp) and species of pheasants and West Mexican Chachalacas (Ortalis poliocephala).

Lice are ectoparasites capable of affecting birds, and can result in direct and indirect damage to their host. Afoxolaner is an isoxazoline that has been shown to be effective against these ectoparasites without known adverse effects. The objective of this research was to evaluate the effect of afoxolaner on lice in pheasants and plain chachalacas. A total of 29 pheasants of different genera and species (Chrysolophus pictus, C. amherstiae, Lophura swinghoii, L. nycthemera, Phasianus colchicus, and Syrmaticus reevesii) and 18 West Mexican Chachalacas (Ortalis poliocephala) naturally infested with Goniodes pavonis were used. The birds were allocated to one of two groups: group 1 treated with 2.50 mg/kg of afoxolaner, and group 2 given no treatment. Ectoparasites were collected using the adhesive tape technique and identified. Afoxolaner was administered later as a single dose to group 1, and the clinical assessment to detect ectoparasites was repeated 28 days post-treatment. On day 28 post-treatment, group 1 was found to be negative for the presence of lice. The body weights were compared at the beginning and end of the clinical assessment in both groups and a significant difference in weight of treated birds was found. The mean body weight decreased by 0.017 g in control group, whereas it increased by 0.016 g in treated group. Oral administration of afoxolaner is an effective option for the treatment of Goniodes pavonis infestations in zoo birds.

Effect of oral afoxolaner on naturally occurring infestations of peacocks by the louse Goniodes pavonis.

BACKGROUND: Ectoparasitism of ornamental birds, including captive species kept in zoos, represents a serious health problem. Up to 13 different species of lice have been reported to affect peacocks worldwide and heavy infestation may cause anaemia. Because of this, alternatives to the prevailing treatments have been sought including use of isoxazolines. This class of drugs has been used successfully in poultry without adverse effects on health or production. OBJECTIVE: To evaluate the effect of afoxolaner on the peacock louse (Goniodes pavonis). ANIMALS: Twenty-three peacocks (Pavo cristatus) with naturally occurring infestation with G. pavonis. METHODS AND MATERIALS: The peacocks were divided in two groups; one was treated once orally with 2.5 mg/kg afoxolaner and the other group received no treatment. Samples were collected using the acetate tape technique, for identification of lice by microscopy. Concomitantly, blood samples were taken to evaluate the haematocrit before and after the intervention. RESULTS: Treatment with afoxolaner significantly decreased the number of peacocks positive for lice (P = 0.02) compared to the control group, in which the number of positive birds did not decrease. The haematocrit improved in the afoxolaner-treated group from a baseline of 46.4%-54.7% at 35 days post-treatment, whereas it decreased in untreated birds (44.6%-40.7%). No adverse effects attributed to afoxolaner treatment were observed. CONCLUSIONS AND CLINICAL IMPORTANCE: Oral administration of afoxolaner is an effective treatment for G. pavonis infestation of peacocks.

Efficacy of two anthelmintic treatments, spinosad/milbemycin oxime and ivermectin/praziquantel in dogs with natural Toxocara spp. infection.

Toxocara canis is one of the most important zoonotic parasites of dogs. The aim of the present study was to compare the efficacy of spinosad/milbemycin oxime and ivermectin/praziquantel in dogs naturally infected with Toxocara spp. We studied 200 dogs with a positive diagnosis of Toxocara spp. Through coproparasitoscopic analysis, two study groups of 100 dogs each were assigned: spinosad/milbemycin oxime at a dose of 30-60mg/kg and 0.75-1.0mg/kg, respectively, or ivermectin/praziquantel administered at a dose of 0.2mg/kg and 5mg/kg, respectively. Both groups received a single dose. Three stool samples, one at day 0 before treatment, and at 14 and 28days post-treatment were examined using concentration-flotation techniques. In both treatments, the number of Toxocara spp. eggs decreased; with spinosad/milbemycin oxime treatment, eggs decreased by 87% at 14days (P=0.008) and 94% at 28days after treatment, compared with 71% at day 14 and 88% at day 28 in dogs medicated with ivermectin/praziquantel. The spinosad/milbemycin oxime treated group showed a greater decrease in the number of Toxocara spp. positive dogs compared to the group receiving ivermectin/praziquantel.